R2R Biofluidics has started on February 1st, 2015. During the next 4 years it aims for the development of a complete process chain for first-time realization of production lines for two selected bioanalytical lab-on-chip devices based on high throughput R2R nanoimprinting in combination with complementary printing and manufacturing technologies. The consortium, consisting of high qualified experts in the fields of engineering and production technology, chemistry and materials development, and biotechnology and life sciences, will fabricate two types of demonstrators by realising a complete high-volume process chain for industrial fabrication of bioanalytical lab-on-chip devices based on setting–up a roll-to-roll (R2R) production line:
Demonstrator 1: In-vitro diagnostic chip with imprinted microfluidic channels based on chemiluminescence detection using photodetectors; containing imprinted optical nanostructures for light coupling and thus improving device performance.
Left: R2R manufacturing of foils with microfluidic patterns for a diagnostic chip. Right: Imprinting of foils with optical effect. The integration of such foils into diagnostic chips allows chemiluminescence based detection with enhanced sensitivity.
Fully foil based microfluidic diagnostic chip for detection of antibiotics resistant bacteria (e.g. methicillin-resistant Staphylococcus aureus, MRSA)
Demonstrator 2: Cell-chips containing imprinted cavities and micro- to nanoscale channels for controlled neuron culturing to be applied in high throughput drug screening.
CFD simulation of capillary driven filling of v-channels for neuron culture plates
Neuron growth master structure on Si wafer, PDMS mold and imprints on foil (filled with blue color and details thereof)
Roll to roll imprinting of neuron growth structure
Time evolution of filling of the neuron growth structure with blue color (for visualisation)
Imprinted neuron growth structure mounted on standard micro titer plate
These types of demonstrators will target application areas, which will clearly benefit from technology advancement in high volume manufacturing, show large potential for commercial exploitation and adopt current standard formats (microtiter plate and microscope slides).